Mimotope ELISA for Detection of Broad Spectrum Antibody against Avian H5N1 Influenza Virus

Mimotope ELISA for Detection of Broad Spectrum Antibody against Avian H5N1 Influenza Virus

BACKGROUND We have raised a panel of broad spectrum neutralizing monoclonal antibodies against the highly pathogenic H5N1 avian influenza virus, which neutralize the infectivity of, and afford protection against infection by, most of the major genetic groups of the virus evolved since 1997. Peptide mimics reactive with one of these broad spectrum H5N1 neutralizing antibodies, 8H5, were identifi...

Standardization of ELISA for Detection of Avian Influenza Virus Antibodies

Evaluation of a competitive ELISA for antibody detection against avian influenza virus

Active serologic surveillance is necessary to control the spread of the avian influenza virus (AIV). In this study, we evaluated a commercially-available cELISA in terms of its ability to detect AIV antibodies in the sera of 3,358 animals from twelve species. cELISA detected antibodies against reference H1- through H15-subtype AIV strains without cross reactivity. Furthermore, the cELISA was ab...

standardization of elisa for detection of avian influenza virus antibodies

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A SPR Aptasensor for Detection of Avian Influenza Virus H5N1

Rapid and specific detection of avian influenza virus (AIV) is urgently needed due to the concerns over the potential outbreaks of highly pathogenic H5N1 influenza in animals and humans. Aptamers are artificial oligonucleic acids that can bind specific target molecules, and show comparable affinity for target viruses and better thermal stability than monoclonal antibodies. The objective of this...